Assistant Professor (Research)

Department of Agriculture and Allied Sciences, Doon Business School, Dehradun, India.

May 2017- May 2019: Senior Research Associate (SRA) under Pool Scientist Scheme (CSIR) in CSIR-National Botanical Research Institute, Lucknow, project entitled “Identification of PGPR induced protein in chickpea under control and stress using comparative subcellular proteomics Approach.”

Scientist Fellow/ Consultant (Jan2013- March 2017): under the major project in “Root biology and its correlation for sustainable agriculture and Soil fertility (RootSF)” in CSIR-National Botanical Research Institute, Lucknow, India.

Senior Research Associate (SRA) under Pool Scientist Scheme (CSIR) in National Institute of Plant Genome Research project entitled “Identification of Heat induced Protein in wheat: A comparative Subcellular Proteomics Approach.”

Post-Doctoral Research Associate in Washington State University (WSU), Prosser, USA. Worked on “Exploring opportunities to increase potatoes protein content and resistance against biotic stress and abiotic stress using molecular approach and germplasm mining at molecular level”.

1999-2001: Received Teaching Assistant Grant by GB Pant University of Agriculture and Technology, Pantnagar, during M.Sc.

2001-2002: Received Research Assistant grant by GB Pant University of Agriculture and Technology, Pantnagar, during PhD.

2001: Received Junior and Senior Research Fellowship conducted by UGC (University Grants Commission) and CSIR (Council of Scientific and Industrial Research) and qualified National Eligibility Test (NET) for Lectureship.

2001: Qualified National Eligibility Test (NET) for Lectureship conducted by Indian Council of Agricultural Research (ICAR) in Genetics

2011: Received Senior Research Associate under Pool Scientist Scheme awarded by CSIR

2012: Received Travel Grant for 11th annual HUPO conference in Boston, Massachusetts, USA from CSIR.

2017: Received Senior Research Associate under Pool Scientist Scheme awarded by CSIR.

Jan2013- May 2019: CSIR SRA (Pool Scientist) /Scientist Fellow under CSIR QHS in major area of root biology.

Accomplishment: Completed proteomics study of rice and chickpea roots in normal and drought condition using 2D Gel Electrophoresis. The comparative proteomics study to see the effect of PGPR on chickpea under normal and stressed conditions was also done. Comparative proteomics study in Vetiver grass for more and good quality essential oil production has also been carrying out. Transcriptomic study of Arabidopsis and Rice root under different stress with and without PGPR is under process. In-vivo and in-vitro Plant-PGPR interaction study was also done to find out the role of PGPR in plant growth and protection.

Dec 2011- Jan 2013: Senior Research Associate (SRA) under pool Scientist Scheme (CSIR) in National Institute of Plant Genome Research project entitled “Identification of Heat induced Protein in wheat: A comparative Subcellular Proteomics Approach.”

Accomplishment: Physiological Screening of nine wheat varieties for high thermo-tolerance and susceptibility under control condition. The cytoplasm and cell-wall proteome of wheat in thermo-tolerant and thermo-sensitive line using 2D Gel Electrophoresis was done.

June 2011-Dec 2011: Post-doctoral Fellow in Indian Agriculture Research Institute (IARI), New Delhi, worked on Draught resistance in wheat and Hybrid development in wheat. 

Oct, 2009- may, 2011: Post-doctoral research associate in Washington State University, USA, worked on Exploring opportunities to increase potatoes protein content and resistance against biotic stress and abiotic stress using molecular approach and germplasm mining at molecular level. 

Accomplishment: Cloning of Prosystemin for over-expression and Silencing in binary vector, Agrobacterium mediated transformation of over-expression and silencing constructs in potato, Molecular analysis, Screening of more than 100 potato germplasms for higher protein, HPLC analysis of more than 50 lines for higher phytonutrient study.

Jan, 2009 – Sept, 2009: Post doctoral fellowship in National Institute of Plant Genome Research, Jawaharlal Nehru University, New Delhi, India. Worked on Potato Proteomics, metabolomics

Accomplishments: Completed the comparative proteomics study of transgenic and wild type potato using 2D Gel Electrophoresis, developed more sweet potato transgenic lines with both constitutive and root specific constructs using Agrobacterium mediated transformation and their molecular characterization.

2002-2008: Worked as Research Scholar at National Institute of Plant Genome Research, Jawaharlal Nehru University, New Delhi, India, in the project entitled "Molecular Mechanism of AmA1 Function in Potato and its Overexpression in Sweet Potato.”

Accomplishments: Establishment of tuber life-cycle proteome and find out the role of ROS pathway during potato tuberization. Comparative proteomics study of transgenic and wild-type potato using 2D Gel Electrophoresis. Domain analysis of AmA1 gene by developing 8 N-terminal and C-terminal deleted AmA1 construct and their transformation in potato. Development of AmA1 construct under root specific B-amylase promoter and constitutive promoter and their transformation in sweet potato.

2001-2002: worked as a research scholar at GB Pant University of Agriculture and Technology, Pantnagar, India as part of PhD.

1999-2001: Worked in the project entitled “Heterosis and combining ability of new plant types (NTP) lines and identification of wide compatible gene in new plant type lines of japonica rice (Oryza sativa L.) ” at GB Pant University of Agriculture and Technology, Pantnagar, India as part of M.Sc. Thesis.

Accomplishments: Development of new superior hybrid lines of NPT rice by crossing of 25 NPT lines and 4 testers.

• Agrawal L., Chakraborty S., Jaiswal, D., Gupta, S., Datta A. and Chakraborty N. (2008) Comparative Proteomics of Tuber Induction, Development and Maturation Reveal the Complexity of Tuberization Process in Potato (Solanum tuberosum L.) J. Proteome Res. 7(9) 3803 - 3817 ISSN: 1535-3893              IF: 5.01                      
• Chakraborty S., Chakraborty N., Agrawal L., Ghosh S., Narula K., Shekhar S., Pande P C., Naik S., Chakraborti S., and Datta A. (2010). Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber. Proc. Natl.  Acad. Sci., USA. 107 (41) 17533-17538                   ISSN:  0027-8424 (Print)  1091-6490 (web)            IF: 9.7 
• Agrawal L, Narula K, Basu S, Shekhar S, Ghosh S, Datta A, Chakraborty N, Chakraborty S. (2013). Comparative proteomics reveals a role for seed starage protein, AmA1 in cellular growth, development and nutrient accumulation. J. Proteome Res.; 12 (11): 4904-30. ISSN: 1535-3893              IF: 5.01
• Shekhar S, Agrawal L, Buragohain, AK, Datta A,  Chakraborty S, Chakraborty N. (2013) Genotype Independent Regeneration and Agrobacterium‐mediated Genetic Transformation of Sweet Potato (Ipomoea batatas L.). Plant Tissue Cult. & Biotech. 23(1): 87‐100. ISSN 1817-3721
• Parmar N, Virdi AS, Singh N, Kaur A, Bajaj R, Rana JC, Agrawal L. & Nautiyal CS. (2014) Evaluation of physicochemical, textural, mineral and protein characteristics of kidney bean grown at Himalayan region. Food Research International, 66, 45–57.  ISSN 0963-9969  (IF: 2.88)
• Garg A, Agrawal L, Misra RC, Sharma S and Ghosh S. (2015) Andrographis paniculata transcriptome provides molecular insights into tissue-specific accumulation of medicinal diterpenes. BMC Genomics. 16:659. doi: 10.1186/s12864-015-1864-y             ISSN: 1471-2164 (IF: 3.99)
• Kumar M, Mishra S, Dixit VK, Agrawal L, kumar M,  Chauhan PS,  Nautiyal CS. (2016). Synergistic effect of Pseudomonas putida and Bacillus amyloliquefaciens ameliorates drought stress in chickpea (Cicer arietinum L.). Plant Signaling & Behavior. 11(1):e1071004. ISSN 1559-2324
• Dixit R, Agrawal L,  Gupta S, Kumar M, Yadav S, Chauhan PS, Nautiyal CS. (2016) 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing Paenibacillus lentimorbus B-30488 controls Southern blight disease in tomato. Plant Signaling & Behavior. 2016;11(2):e1113363  ISSN 1559-2324
• Kumar S, Chauhan PS, Agrawal L, Raj R, Yadav S, Singh PC, Raj SK, Nautiyal CS. (2016) Paenibacillus lentimorbus-30488 inoculation enhances tobacco growth and extenuating the virulence of Cucumber mosaic virus. PLoS ONE 11(3): e0149980   ISSN: 1932-6203 (IF: 3.23)
• Shekhar S, Agrawal L, Mishra D, Buragohain A, Unnikrishnan M, Mohan, Chakraborty S, and Chakraborty N. (2016) Ectopic expression of amaranth seed storage albumin modulates photoassimilate transport and nutrient acquisition in sweetpotato. Sci Rep 2016 5;6:25384  ISSN: 2045-2322   IF: 5.56
• Agrawal L, Gupta S,  Mishra SK,  Pandey G,  Kumar S,  Chauhan P S, Chakrabarty D and  Nautiyal CS. (2016) Elucidation of complex nature of PEG induced drought-stress response in rice root using comparative proteomics approach. Front. Plant Sciences, 7: 1466  Electronic ISSN: 1664-462X IF: 4.495
• Divya Mishra, Shubhendu Shekhar, Lalit Agrawal, Subhra Chakraborty, Niranjan Chakraborty (2016) Cultivar-specific high temperature stress responses in bread wheat (Triticum aestivum L.) associated with physicochemical traits and defense pathways. Food Chemistry 221, 1077–1087  ISSN: 0308-8146  IF: 4.03
• Srivastava A.*, Agrawal L*, Kumar S, et al (2017) Ageratum enation virus infection induces programmed cell death and alters metabolite biosynthesis in Papaver sominiferum. Front Plant Sci. 2017; 8: 1172.   Electronic ISSN: 1664-462X IF: 4.495
•  Raj R, Kaur C, Agrawal L, Chauhan PS, Kumar S, Raj SK (2018)  Full-length genome sequence of Cyrtanthus elatus virus-A isolated from Narcissus tazetta in India 3 Biotech 8(3):168 IF: 1.495
• Srivastava S, Srivastava S,  Agrawal L,  et al (2018) Chlorella vulgaris and Pseudomonas putida interaction modulates phosphate trafficking for reduced arsenic uptake in rice (Oryza sativa L.) Jour Hazz Materials. 351:177-187                 IF: 6.434
• Dixit R*, Agrawal L*, Singh S, Singh, Prateeksha, Singh P, Prasad V, Chauhan P. (2018) Paenibacillus lentimorbus B-30488 induces autophagy to protect tomato from necrotrophic fungus Sclerotium rolfsii. Microbio. Res 215, 164–174.   IF: 3.7
• Kumar S*, Agrawal L*, Srivastava A, Chauhan PS, Raj SK, (2018) Study of biochemical and histopathological changes induced in the sweet pepper (Capsicum annuum L.) in response to Chilli leaf curl virus infection. Physiological and Molecular Plant Pathology 104, 95-102
• Kuar C., Raj R, Agrawal L, Chauhan PS, Kumar S, Raj SK (2019) Elimination of Bean yellow mosaic virus from infected cormels of three cultivars of gladiolus using thermo, electro and chemotherapy. 3 biotech 9:154                IF: 1.495
• Chauhan PS, Lata C,  Tiwari S, Chauhan AS,  Mishra SK, Agrawal L, Chakrabarty D, Nautiyal CS (2019) Transcriptional alterations reveal Bacillus amyloliquefaciens-rice cooperation under salt stress Sci Rep., 9:11912         IF: 4.2
• Raj R, Agrawal L, Chauhan PS, Kumar S, Raj SK (2019) Complete genome sequence analysis of   Narcissus yellow stripe virus associated with leaf stripe symptoms on Narcissus tazetta in India. 3 biotech   9:409    IF: 1.495
•  Chauhan R, Awasthi S, Indoliya Y, Chauhan AS,  Mishra S, Agrawal L, Srivastava S, Dwivedi S, Singh PC, Mallick S, Chauhan PS, Pande V, Chakrabarty D, Tripathi RD (2020)  Transcriptome and proteome analyses reveal selenium mediated amelioration of arsenic toxicity in rice (Oryza sativa L.)” accepted in Journal of Hazardous Materials ,   IF: 7.6
• Gupta S, Mishra SK , Misra S,  Pandey V, Agrawal L#, Nautiyal CS ,  Chauhan PS (2020) Revealing the complexity of protein abundance in chickpea root under drought-stress using comparative proteomics approach. Accepted in  Pl Physiol and Biochem (PLAPHY-D-19-02431)
• Madhuree Kumari, Shipra Pandey, Shashank K Mishra, Ved P Giri, Lalit Agrawal, Sanjay Dwivedi, Alok Pandey, Aradhana Mishra and Chandra S Nautiyal (2020) Omics based mechanistic insight into the role of bioengineered nanoparticles for biotic stress amelioration by modulating plant metabolic pathways (Accepted in Front of Bioeng. Biotech. Manuscript ID: 524432)

• P Tiwari, D Srivastava, A S Chauhan, Y Indoliya, P K Singh, Shalini Tiwari , Touseef Fatima, Shashank Kumar Mishra, Sanjay Dwivedi, Lalit Agrawal, Poonam C Singh , Rudra D. Tripathi , P. A. Shirke , D Chakrabarty, Puneet S Chauhan, Chandra Shekhar Nautiyal (2020) Root system architecture, physiological analysis and dynamic transcriptomics unravel the 2 drought-responsive traits in two contrasting rice genotypes. Submitted in Pl Physiol and Biochem (PLAPHY-D-20-00148)
• Rashmi Raj, Charanjeet Kaur, Lalit Agrawal, Susheel Kumara, P. S. Chauhan, and S. K. Raj (2020) Development of an efficient protocol for elimination of Cyrtanthus elatus virus from Narcissus tazetta explants through in vitro chemotherapy, electrotherapy alone and in combination. Submitted in Jour of Virol method (VIRMET_2020_14)
• Agrawal L, Mishra SK *, Gupta S *, Kumar S, Chauhan PS, Lavania UC, Nautiyal CS (2020) Proteomic reference map of vetiver root (Chrysopogon zizanioides L. Roberty) and its comparison with contrasting root architectural variety illustrates pathway abundance proteins
• L Agrawal*, SK Mishra*, S Gupta, G Pandey, S Kumar, A Bhatia, V Pandey, RD Tripathi, PS Chauhan, CS Nautiyal (2020) Drought-responsive reversible and irreversible changes in the cytoplasmic proteins: comparative proteomics of rice genotypes with contrasting tolerance

• Lalit Agrawal  et al., (2018) Drought-responsive reversible and irreversible changes in the cytoplasmic proteins: comparative proteomics of rice genotypes with contrasting tolerance. VIth Internation Conference on plant and environmental pollution: ICPEP-VI, 27-30 Nov 2018, CSIR-NBRI, Lucknow India
•  Lalit Agrawal  et al., (2016) Proteomic reference map of vetiver root (Chrysopogon zizanioides L. Roberty) and its comparison with contrasting root architectural variety illustrates pathway abundance proteins. PSI-AOAPO International Conference on functional and interactive Protemics: Application in food and health. 14-17 Dec, 2016, New Delhi, India
• Lalit Agrawal et. al., (2016) Ageratum enation virus infection in Papaver sominiferum disorders plant's architecture, affects alkaloid and metabolite biosynthesis, and accelerates programmed cell death. APSF-2016 26th-27th November, 2016, Shahjahnpur, UP, India
• Kumar M., Mishra S., Dixit VK., Kumar M., Agrawal L., Khare P., Chauhan PS. (2016) 37th Annual meeting of PTCA (India) and National Symposium on Plant Biotechnology for Crop Improvement. 25-27 Feb 2016, CSIR-NBRI, Lucknow, India
• Srivastava S., Tondon A., Bist B., Tiwari S., Kumar M., Shivhare R., Agrawal L., Lata C., Chauhan PS., Nautiyal CS. (2015) Plant growth promoting pseudomonas mediates stress amelioration. Vth Internation Conference on plant and environmental pollution: ICPEP-V, 24-27 Feb 2015, CSIR-NBRI, Lucknow India
•  Misra S, Gupta S, Mishra SK, Lalit Agrawal, et al (2014) Profiling of gene expression through microarray analysis influenced by plant growth promoting rhizobacteria, Pseudomonas putida MTCC5279 in Arabidopsis thaliana National Workshop on Advances in PGPR Research October 7-8, 2014 Banaras Hindu University, Varanasi 221005, India
• Pooja Verma, Sumit Yadav, Lalit Agrawal, Puneet Singh Chauhan and Chandra Shekhar Nautiyal (2013) Rhizosphere colonization controlled by purB gene in Pantoea agglomerans (2013) “National Conference on Microbes Promoting Crop Health, Productivity & Sustainability (26-27 October, 2013)” jointly organized by CSIR-CIMAP & CSIR-NBRI at Lucknow
• Lalit Agrawal, Shubhendu Shekhar, Sanjay K. Gupta, Niranjan Chakraborty (2012). Comparative Proteomics Analysis of Cold Tolerant and Susceptible Potato Tuber in Response to Cold Storage.  HUPO 11th Annual World Congress, September 9 – 14, 2012. Boston, Massachusetts, USA.
• Lalit Agrawal and Duroy Navarre (2010). Exploring opportunities to increase potatoes protein content and disease resistance using molecular methods or germplasm mining. PAA 94th Annual conference Aug15-19, 2010. Corvallis, Oregon State University, USA.
• Lalit Agrawal, N Chakraborty, A Datta, and S Chakraborty (2009) Comparative Tuber Proteome in Wild-Type and Transgenic Potato Expressing a Seed Albumin. HUPO 8th Annual World Congress, September 26 – 30, 2009. Toronto, Canada
•  Lalit Agrawal, Subhra Chakraborty, et al (2009) Plant & Animal Genomes XVII Conference. January 10-14, 2009. Town & Country Convention Center San Diego, CA
• L. Agrawal, S. Chakraborty, A. Datta, and N. Chakraborty (2007) Differential Display of Potato Proteomes Reveals Predicted and Unexpected Proteins during Tuberization. HUPO 6th Annual World Congress, October 6–10, 2007, Seoul, Korea
• Rajgourab Ghosh, Sudip Ghosh, Lalit Agrawal, Niranjan Chakraborty, Asis Datta, and Subhra Chakraborty (2009) Comparative proteome analysis of differentially expressed proteins in wild type and genetically engineered crop plants for patho-stress and improved nutritional quality. The 6 Solanaceae Genome Workshop th th 8 -13 November, 2009 Le Meridien, New Delhi, India.
• Lalit Agrawal, Shubhendu Shekhar, Subhra Chakraborty, Asis Datta, and Niranjan Chakraborty (2009) Comparative analysis of alterations in potato (solanum tuberosum l.) during tuber induction, development and maturation. The 6 Solanaceae Genome Workshop th th 8 -13 November, 2009 Le Meridien, New Delhi, India.
• Arnab Chattopadhyay, Aarti Pandey, Deepti Bhushan, Lalit Agrawal, Asis Datta, Subhra Chakraborty and Niranjan Chakraborty (2008) Dehydration stress responsive comparative proteome of Lathyrus sativus, a hardy legume HUPO 7th Annual World Congress, Aug 16 – 20, 2009. Amsterdam, Netherlands.